Single cell analysis is essential to detecting and understanding cancer heterogeneity.
The Epic Sciences' platform analyzes all nucleated cells within a blood sample at single cell resolution. Cells from a patient’s blood sample are deposited on replicate glass slides and compared for morphological features, expression of biomarkers and nuclear integrity, using immunofluorescent staining. Guided by cell phenotype and marker expression, CTCs are individually recovered from the slide surface. Their genomes are amplified (WGA) and analyzed by next generation sequencing (NGS) for the presence of point mutations, copy number alterations, genome wide chromosomal instability, ploidy or genome wide scarring.
Epic Sciences’ CNV assay service consists of:
Example data showing the clonal evolution. CTCs from a single patient were identified and characterized through our CNV assay. The distinct clonal species and their relationship to each other are shown in the figure above.
Epic Sciences’ targeted resequencing assay service consists of:
Comparison of called variant function (left) and impact (right) across all patient CTCs
Learn more about our genomics assays are being used: